Now neuroscientists have "enough evidence to say that the growth and development of nervous tissue (neurogenesis) is a target of choice if we want to improve memory formation or mood, or even prevent the decline associated with aging, or associated with stress" (Sandrine Thuret).
"Japanese scientists have shown that actually soft diet impairs growth and development of nervous tissue, as opposed to food that requires mastication — chewing — or crunchy food." ( https://www.ted.com/talks/sandrine_thuret_you_can_grow_new_brain_cells_here_s_how )
Due to the complexity of the swallowing process, many adverse health conditions can influence swallow function. Swallowing disorders may occur because of wide variety of neurological and non-neurological conditions such as oropharyngeal or esophageal cancer, neurologic diseases such as stroke, traumatic brain injury, spinal cord injury, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS) and Alzheimer’s disease. Stroke is the leading cause of neurologic dysphagia. “Common complications of dysphagia in both stroke and dementia include malnutrition and pneumonia” (Sura, L. 2012).
Exercising with FacExer combines isotonic and isometric exercises for all groups of muscles of your head, face and neck, including muscles of mastication (chewing).
The main muscles involved in chewing are masseter, temporalis, medial and lateral pterygoids.Good news from FACEXER team : in 2017 we started production of FACEXER from the stronger material, which withstands moderate human chewing pressure (similar pressure we are using to bite of an apple).
Exercises with FACEXER to target muscles of mastication will be published in our next blog.
The following article from "International Journal Of Medical Science" "Chewing Maintains Hippocampus-Dependent Cognitive Function" provides information about importance to exercise muscles of mastication (chewing) to preserve cognitive function.
Chewing Maintains Hippocampus-Dependent Cognitive Function
Chen H, Iinuma M, Onozuka M, Kubo KY. Chewing Maintains Hippocampus-Dependent Cognitive Function. Int J Med Sci 2015; 12(6):502-509. doi:10.7150/ijms.11911. Available from http://www.medsci.org/v12p0502.htm
Huayue Chen1*, Mitsuo Iinuma2, Minoru Onozuka3, Kin-Ya Kubo4*
- Department of Anatomy Gifu University Graduate School of Medicine, Gifu, 501-1194, Gifu, Japan
- Department of Pediatric Dentistry, Division of Oral Structure, Function and Development, Asahi University, School of Dentistry, Mi- zuho, 501-0296, Gifu, Japan
- Department of Judo Therapy and Medical Science, Faculty of Medical Science, Nippon Sport Science University, Yokohama 227-0033, Kanagawa, Japan
- Seijoh University Graduate School of Health Care Studies, Tokai, 476-8588, Aichi, Japan
* Corresponding author: Dr. H. Chen, Department of Anatomy Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Gifu, Japan. Phone: +81 58 230 6295; Fax: +81 58 230 6298; Email: email@example.com. Dr. Kin-ya Kubo, Seijoh University Graduate School of Health Care Studies, Tokai, 476-8588, Aichi, Japan. Email: firstname.lastname@example.org
Mastication (chewing) is important not only for food intake, but also for preserving and promoting the general health. Recent studies have showed that mastication helps to maintain cognitive functions in the hippocampus, a central nervous system region vital for spatial memory and learning. The purpose of this paper is to review the recent progress of the association between mastication and the hippocampus-dependent cognitive function. There are multiple neural circuits connecting the masticatory organs and the hippocampus. Both animal and human studies indicated that cognitive functioning is influenced by mastication. Masticatory dysfunction is associated with the hippocampal morphological impairments and the hippocampus-dependent spatial memory deficits, especially in elderly. Mastication is an effective behavior for maintaining the hippocampus-dependent cognitive performance, which deteriorates with aging. Therefore, chewing may represent a useful approach in preserving and promoting the hippocampus-dependent cognitive function in older people. We also discussed several possible mechanisms involved in the interaction between mastication and the hippocampal neurogenesis and the future directions for this unique fascinating research.
Key words: Cognition, Hippocampus, Mastication
With the rapid increase in the elderly populations, dementia has become an increasingly important health and socioeconomic issues . Cognitive deficits are associated with aging as well as neurobiological, psychological and social factors [2, 3]. Recently, attention has focused on the oral health conditions, especially mastication, as well as on the number of teeth, in the context of cognitive deficits. Both animal and human studies suggested a possible causal relationship between mastication and cognitive function [4-6]. Many older people have masticatory problems due to tooth loss, which compromises general health status. Some foods become difficult to eat for older people, most notably when tooth loss is present . Loss of masticatory function is also associated with increased disability and mortality . Consequently, being able to chew properly is of utmost important for elderly to maintain a healthy diet and preserve cognitive function. It has been demonstrated that mastica- tion is of great importance for peripheral sensory input to the hippocampus for preserving and promoting the cognitive function. The systemic effect of the masticatory dysfunction is suggested to be an epidemiologic risk factor for dementia [9, 10]. The importanceof the cognitive modulation by the hippocampus is now widely recognized. Hippocampus is important for the formation and retrieval of episodic memories in humans . Accumulating evidence indicates that impaired masticatory function causes morphological and functional alterations of the hippocampus [12-20]. Mastication or chewing helps to maintain the hippocampal function [20-23]. In the present manuscript, we aim to provide a comprehensive review, and a critical analysis of the current understanding of the relationship between mastication and the hippocampal functions.
Functional analysis of masticatory apparatus
The major masticatory components are bones, muscles, teeth, and soft tissues. Bones involved in mastication are maxilla and mandible. Mastication movements are executed using muscles connected to the maxilla and mandible. The mandible and maxilla are the anchor points for the deciduous or primary teeth in children and permanent teeth in adults. The soft tissues, including tongue, lips and cheeks, are also important in the manipulation of food during oral processing . Mastication is the first stage of the digestion and involves the intermittent rhythmic act in which the tongue, facial and jaw muscles act in coordination to position the food between the teeth, cut it up and prepare it for swallowing. Mastication aims to decrease particle-size distribution in the food bolus and forms a cohesive bolus with the saliva in order to facilitate swallowing . Teeth provide a uniquely discriminating sense of touch and directional specificity for occlusal awareness, intra-oral contact for management of a food bolus, discrimination of food texture and hardness, and control of jaw muscles for mastication and swallowing . These specific features of teeth are closely linked with periodontal sensitivity suggesting an integrated role of the pulp-dentine-enamel complex . These data further define the importance of teeth in the sensorimotor control of jaw function. The neural basis of the pulpal sensitivity contributes to the exquisite sensory discrimination mechanism of teeth. Soma- tosensory information from orofacial mechanorecetors, such as periodontal, mucosal, muscle spindle, thermoreceptor, and gustatory and olfactory receptor, plays a critical role in the generation and control of jaw movements during mastication [25-27].
There are several methods to study mastication in animals and humans. In animal experiments, molar extraction [13, 14], occlusal disharmony or bite-raised condition [16, 17], soft-diet feeding [28, 29] and chewing wooden sticks [21, 22] are common used for examining masticatory functions. In human studies, the independent variables include self-reported dental status and chewing difficulty [9, 30]. The oral cavity examination is a standardized protocol with regard to the number of natural teeth, occlusion and periodontal conditions.
The neural circuits between masticatory organs and hippocampus
A series of studies have demonstrated the contributions of various cerebral cortical areas for mastication . Epidemiological research showed that loss of residual teeth, inadequate prostheses and a decrease in biting force are directly associated with the worsening of dementia [9, 30]. In animal studies, mastication increases cerebral cortical blood flow and widely activates various cortical areas of the soma- tosensory, supplementary motor and insular cortices . Blood oxygen levels in the prefrontal cortex and the hippocampus are increased by mastication, which may be essential for learning and memory processes . Masticatory stimulation may prevent degradation of senile brain function and stress-related disorders without medication. The decrease of masticatory function, by either extraction of or reduction in molars and associated long-term soft-diet feeding, can inhibit learning and memory [12, 13, 16, 18, 19, 22, 33, 34]. Learning ability was shown to decrease in adult rats from 2 to 30 months after the extraction of molars [13, 28, 29]. Soft-diet feeding also suppresses spatial learning ability from 6 to 12 months [28, 29, 35]. Spatial learning deteriorates after a period without molars longer than 2 months in young adult animals [33, 34]. Therefore, it is important to understand the relationship between mastication and brain function with aging.
The sensory information from the oral cavity including teeth is transmitted through the trigeminal sensory nerve to the trigeminal sensory nuclei, cerebellum, hypoglossal motor nuclei and the brainstem reticular formation [27, 31, 36, 37]. The reticular for- mation and the ascending reticular activating system are necessary for arousal of the brain for attention, perception and conscious learning. The neurons of the trigeminal sensory nuclei reach to the ventral posterior thalamic nucleus, the reticular formation and hypothalamus. The sensory information from the ventral posterior thalamus nucleus terminates on the somatosensory cortex. The neurons of the somatosensory cortex project their axons to the somatosensory association area, which has reciprocal projection with the entorhinal cortex. The entorhinal cortex is a major afferent source to the hippocampal dentate gyrus (DG). Therefore, the sensory information from the masticatory organs may affect hippocampus via thalamus and cerebral cortex. Hypothalamus receives input from the reticular formation and projects to hippocampus directly as opioidergic and histaminergic fibers. Hippocampus plays a vital role in controlling the release of various hormones by the hypothalamic-pituitary-adrenal (HPA) axis. Corticotrophin-releasing hormone (CRH) produced by the hypothalamus stimulates the secretion of adre- nocorticotropic hormone (ACTH) from the anterior pituitary, which stimulates the release of corti- costerone from the adrenal cortex [6, 38]. Due to the lipophilic nature, corticosterone can achieve rapid access to the brain. Hippocampus shows the highest density of glucocorticoid receptors (GR), making it a target for stress hormone actions . Hippocampus receives projections of noradrenergic, serotonergic and dopaminergic fibers from the locus coeruleus, raphe nuclei and the ventral tegmental area, which are a part of the ascending reticular activating system. Thus, it is possible that mastication can influence the hippocampal function through the reticular formation. Therefore the effects of mastication on hip- pocampus may be attributable to multiple neural pathways.
Hippocampal functional morphological changes caused the masticatory dysfunction
Evidence from neuroimaging studies suggests that masticatory dysfunction induced spatial memory and learning deficits associated with structural and functional reorganization of the hippocampus. Pre- vious studies have elucidated the possible link be- tween masticatory dysfunction and the hippocam- pus-dependent cognition using animal models, such as molar teeth extraction, crown reduction or bite el- evation [14-18]. These animal models are able to chew, but the occlusal function markedly decreased, causing the degenerative changes in the periodontal mecha- noreceptors, suggesting a suppression of sensory stimulation from the periodontal ligaments during chewing. In these experiments, molarless or bite-raised animals showed spatial memory deficits with the hippocampal morphological alterations .
1. Hippocampal pathological morphology and masticatory dysfunction
Animal studies have found that aged mice or rats with memory impairments have higher activity of the HPA axis [17-20]. HPA axis activity is negatively associated with both spatial memory performance and the hippocampal neurogenesis. Reduced masticatory stimulation decreased the hippocampal volume and induced the memory deficits. The pyramidal cell density of the hippocampal Cornu Ammonis 1 (CA1) and CA3 regions in the molarless or bite-raised
mice was significantly decreased [12, 13, 18]. The number of dendritic spines reduced and the dendritic branching declined in an age-dependent manner [18, 20]. The dendritic spines in the hippocampus are al- tered by masticatory dysfunction through intracellu- lar mechanisms that reorganize the neuronal cyto- skeleton by producing cytoskeletal changes [40, 41]. Mice fed with a soft diet exhibited lower densities of synaptophysin- immunoreactive terminals and syn- aptic formation in the hippocampus .
Neurogenesis is well-established to occur in the subgranular zone (SGZ) of the hippocampal DG and the forebrain subventricular zone (SVZ) throughout life . DG is typically considered to have a key role in the encoding of spatial and contextual information, particularly in pattern separation and novelty detec- tion . The newly generated neurons in the SGZ migrate to the inner granule cell layer, rapidly extend long axonal projections along the mossy fiber path- way and reach their target CA3 pyramidal neuronal cell layer, form connections with the CA3 neurons, hilar interneurons and release glutamate as their main neurotransmitter thus attaining a functional signifi- cance. Newly generated cells in the adult mouse hip- pocampus are found to exhibit neuronal morphology and display passive membrane properties, action po- tentials and functional synaptic inputs similar to those found in the mature DG cells. The rate of neurogene- sis is modulated by various physiological and patho- logical conditions. The newly generated cells may have a function in cognition and brain repair mecha- nisms. Animal studies showed that prolonged masti- catory dysfunction suppresses the neurogenesis of DG granule neurons . The neurogenesis both in the SVZ and the hippocampal DG decreased in the soft-diet-fed mice . Molarless or bite-raised condi- tion in mice and rats significantly decreased cell pro- liferation in the hippocampal DG in an age-dependent manner [15, 21]. After extracting molar teeth or raising the bite in aged mice, the cell proliferation decreased abruptly, and then increased, but did not recover to the control levels. These findings suggest that reduced mastication impairs cell proliferation in the hippo- campal DG, leading to spatial learning and memory deficits.
1. Hippocampal molecular morphology and masticatory dysfunction
The prototypical immediate early gene c-fos is transcribed in neurons within minutes after stimulation by various depolarizing and neurotrophic intercellular signals, and rapid degradation of c-fos mRNA ensures that its expression represents recent changes in neuronal activities . Fos protein is an indicator of the neural plasticity and is strongly implicated in memory formation. Experience and spatial learning stimulate Fos expression. It was demonstrated that c-fos knockout mice exhibited deficits in long-term memory and synaptic plasticity . Moreover, memory impairment following brain ischemia is often associated with decreased c-fos expression . Masticatory dysfunction caused a reduction in the number of Fos-positive cells in CA1 region. This effect was more pronounced the longer the molarless condition persisted. The suppression of Fos induction in CA1 region induced by the molarless condition was reduced by restoring the lost molars with artificial crowns .
The cholinergic system of the basal forebrain is one of the key transmitter systems for learning and memory. Hippocampus receives abundant regulatory inputs from the basal forebrain cholinergic system. The hippocampal cholinergic system plays a crucial role in spatial learning . Treatment with donepezil, a potent and selective acetylcholinesterase inhibitor, significantly enhanced the survival of newborn neurons. Donepezil treatment could reverse the chronic stress-induced decrease in neurogenesis. Activation of the cholinergic system promotes sur- vival of newborn neurons in the adult DG under both normal and stressed conditions . Administration with neurotoxin 192 IgG-saponin selectively de- stroyed cholinergic neurons of basal forebrain, im- paired neurogenesis in the granule cell layers and increased apoptotic cell numbers specifically in the subgranular zone of DG region . The pro-cognitive influences of the cholinergic system in the hippo- campus involved the production of new neurons, which contribute to DG synaptic plasticity and memory trace formation. Targeting the cholinergic system with cholinesterase inhibitors, which leads to cognitive improvement in patients afflicted with age-related dementia, could possibly exert its thera- peutic action through stimulation of hippocampal neurogenesis [51, 52]. In the molarless or bite-raised aged mice, hippocampal acetylcholine release, acetyl- transferase and choline acetyltransferase activity were significantly decreased [13, 53]. The number of choline acetyltransferase-immunopositive neurons in the medial septal nucleus was also reduced. However, the molarless or bite-raised condition had little effect in young mice, indicating an age-dependent decrease in the hippocampal cholinergic system. Reduced masti- catory activity in mice may be involved in the devel- opment of age-related functional impairment of the hippocampal cholinergic system.
Recent studies showed that impairment of mas- ticatory function downregulated memory-related signaling pathways and genes in the hippocampal neurons, including brain-derived neurotrophic factor
(BDNF) and its receptor, tropomyosin-related kinase B (trkB) [54, 55]. BDNF is a family of nerve growth factors which bind to trkB. In response to BDNF sig- naling, its specific high-affinity receptor trkB has an enhancing effect on nerve transmission. Both BDNF and trkB have been shown to be important modula- tors of synaptic plasticity and are critical to the de- velopment of the central nervous system . Animal experiments have demonstrated that BDNF is re- quired for the maintenance of dendritic spines in the adult brain . BDNF–TrkB signaling can influence dendritic spine and synapse density in hippocampal neurons . The expression of trkB-mRNA has been used as an effective marker for elevated synaptic transmission levels in the signaling pathways related to spatial learning. The trkB-mRNA levels were sig- nificantly lower in tooth extraction rats [56, 57]. The spatial memory and the number of trkB-mRNA-positive cells were both negatively af- fected by the duration of tooth loss and the number of teeth extracted. As a mediator of the hippocam- pal-dependent learning and memory, the BDNF-trkB binding plays a critical role in activity-dependent synaptic plasticity. The spatial memory impairment in rats could have a close relationship with the decrease in the trkB levels of the pathways located from the trigeminal nerve area to the hippocampus.
2. Hippocampal astrocyte and masticatory dysfunction
Astrocytes undergo modification, leading to hypertrophy and hyperplasia, increasing the produc- tion of pro-inflammatory cytokines, such as microgli- al-derived IL-1b. The hypersecretion of immuno- modulating cytokines and the consequent inflamma- tory responses in the astrocytes and neurons are fre- quently observed in aged hippocampus, attributed to age-related cognitive impairment . Astrocytes play a central role virtually in all forms of neuropa- thology, determining, to a large extend, the progres- sion and outcome of neurologic diseases . Glial fibrillary acidic protein (GFAP) is considered to be a universal glial marker and is generally acknowledged as a sign of pathological response of astroglia, the reactive astrogliosis. GFAP-positive astrocytes vary between brain regions, with about 80% of cells in the hippocampus . Aging is associated with astrocyte proliferation and increase in the expression of GFAP, both considered to be signs of reactive astroglial hy- pertrophy . Molarless or bite-raised mice or rats showed hypertrophy of astrocytes in CA1, suggesting glial cells are inflamed and degenerating, implying that the production of cytokines is increased [4, 14]. The number of GFAP-positive astrocytes increased with aging, especially in molarless or bite-raised mice.
Masticatory deficiency not only caused neuronal de- generation, but also induced an increase of astrocyte number accompanied by morphological and physio- logical changes of the hippocampus.
Sensory input from the masticatory organs is essential to maintain the hippocampus-dependent learning. Masticatory impairment of the young ani- mals may not affect the hippocampus in the short term, as the hippocampus receives various peripheral sensory inputs continuously to maintain its function. In aged animals, however, reduced masticatory activ- ity and degenerative periodontal mechanoreceptors do not provide sufficient sensory input to maintain the hippocampal function, leading to impairment of the hippocampal cognitive performance. For young mice, a period longer than two months is required to decrease the learning ability. Aged molarless or bite-raised mice rapidly developed the learning defi- cits [4, 6, 14, 19].
Chewing stimulation helps to maintain the hippocampal function
Several human studies have showed that chew- ing is effective for preserving hippocampal function, which deteriorates with aging [9, 62-66]. Based on large-scale survey data from several European coun- tries, Listl provided novel epidemiological evidence supportive of an association between oral health and cognitive functioning . A population-based cross-sectional study suggested that natural teeth are important for the hippocampus-based cognitive pro- cesses, such as episodic long-term memory . Chewing or even sucking a piece of sugar-free, spearmint flavoured gum was shown to be associated with immediate better cognitive performance, im- proved the score of word recall, sensitivity index of the spatial working-memory task, and reaction time of the numeric working-memory task in humans. Chewing increased the cerebral blood flow and might decrease the risk of cognitive impairments [62-64]. An association between chewing ability and cognition was found in a population-based study. Rural com- munity residents aged 70-74 without dementia, hav- ing poor chewing ability were found to perform worse on the same cognitive tests . In another study, the association between chewing gum and cognitive performance depended on individual dif- ferences such as feeling thirsty and being an introvert . Lexomboon and colleagues conducted an inter- esting study concerning the relationship between chewing ability, tooth loss, and cognitive impairments in a general Swedish older population . They found that the association between chewing ability and cog- nitive impairments was stronger than that between tooth loss and cognitive impairment when adjusted
for age, sex, and education. They suggested that chewing itself may positively influence cerebral blood flow, alleviated stress, and therefore enhance cogni- tive ability. Recently, Brobeil et al. reported that pro- tein tyrosine phosphatase interacting protein 51 (PTPIP51) is probably a new protein regulating memory formation by influencing dendritic and ax- onal growth and synaptogenesis . The expression of PTPIP51 was specifically restricted to the cerebel- lum and the hippocampus , suggesting the inter- action between cerebellum and hippocampus. Func- tional magnetic resonance imaging (fMRI) study re- vealed that chewing affects the cerebellar functions . Cerebellum may be involved in chewing rhyth- micity and motor control motivated by proprioceptive inputs. The increase in the cerebellar neural activity during chewing may improve the hippocam- pus-dependent memory and cognition.
In animal experiments, mice fed a soft diet showed low neurogenesis, while mice fed only a hard diet or a hard diet after a soft one showed normal or recovered neurogenesis . This study suggests that feeding with a hard diet improves the neurogenesis. Chewing under restraint condition reversed the stress-induced suppression of cell proliferation in the DG [6, 21]. Chewing during the stressful condition attenuated the effect of stress on cognitive function. Chewing during a stress event altered the HPA axis function and improved the ability to cope with stress in rodents [21, 70-73]. Chewing during restrain stress significantly suppressed the stress-induced en- hancement of CRH expression in the paraventricular nucleus . The number of stress-suppressed GR-immunopositive neurons in the hippocampal CA1 region was increased after chewing stimulation . GRs play an important role in memory consoli- dation and retrieval . Neuroendocrine activation of the HPA axis is counteracted by GR-mediated negative feedback, which terminates the stress re- sponse. Masticatory dysfunction inhibited the nega- tive-feedback response by downregulation of GR protein and mRNA expression . In aged mice, the molarless or bite-raised condition suppressed cell proliferation in the hippocampal DG [15, 21]. Pre- treatment with metyrapone, the corticosterone syn- thesis blocker, inhibited the increase in plasma corti- costerone level induced by the bite-raised condition, and also attenuated the reduction in cell proliferation . Administration of metyrapone attenuated the chronic stress-induced neuronal degeneration in the hippocampus and chronic stress-induced impair- ments in spatial learning in rats . Immobilization stress suppressed cell proliferation in hippocampal DG region. However, chewing under restraint stress blocked the stress-induced suppression of cell prolif-
eration in the DG region. The functional morphologic deficits induced by the molarless condition in aged SAMP8 mice was a result of increased plasma corti- costerone levels, and that chewing under restraint stress prevented the stress-induced suppression of the neurogenesis in the DG, which is required for the hippocampus- dependent learning and memory in adults. Thus, chewing during stress event may at- tenuate stress-induced impairment in cognitive func- tion.
Chewing during stress in rats and mice attenu- ated stress-induced increase in corticosterone level, CRH expression, c-Fos protein induction, phosphor- ylation of extracellular signal-related protein kinase 1/2, oxidative stress and nitric oxide in the hypo- thalamus. Chewing also attenuates stress-induced impairments of plasticity in the hippocampus by ac- tivating stress-suppressed N-methyl-D-aspartate (NMDA) receptor-mediated long-term potentiation (LTP), which is responsible for learning and memory . Stress caused prolonged activation of the HPA axis, stimulating adrenal cortex to secrete corti- costerone. Increased corticosterone level caused a Ca2+ influx into neurons via activation of corticosterone receptors, attenuated cellular excitability and weak- ened hippocampal LTP selectively that depends on NMDA receptor [71, 77]. Stress-attenuated hippo- campal plasticity could be counteracted when the rats have an opportunity to behaviorally respond to the stress by chewing a wooden stick. Chewing under stress ameliorated stress-induced hippocampal im- pairment by rescuing NMDAR function [71, 77]. Chewing also could induce histamine release and stimulate histamine H1 receptor activation in the hippocampus. Chewing-induced activation of the histaminergic nervous system in the hippocampus facilitated the extracellular histamine level and re- stored stress-attenuated NMDA receptor function in the hippocampus.
Chewing could reverse the HPA axis function, improve the ability to cope with stress and alleviate chronic stress-induced hippocampus-dependent spa- tial learning deficits. Effects of chewing on the hip- pocampal function may be attributable to the multiple pathways, including various regulatory nervous in- puts, memory-related genes and signaling pathways, stress hormone and its receptors. It was reported that gum chewing improved the performance of memory recall in elderly subjects, but did not show any effects in the young adult subjects [4, 78]. As compared with young animals, chewing is more effective on the aged animals by supplying additional sensory inputs to improve hippocampal function, which deteriorates with aging.
Conclusions and the future directions
Mastication plays an important role in preserv- ing the hippocampus-dependent cognitive function. Masticatory deficiency impairs the hippocampal morphology via the neural circuits and HPA axis, resulting in spatial memory and learning deficits. Mastication during stress event could reverse stress-induced neuronal degeneration and suppres- sion of cell proliferation in the hippocampus and at- tenuates stress-induced impairments of plasticity in the hippocampus by various regulatory nervous in- puts, memory-related genes and signaling pathways, stress hormone and its receptors. Therefore, chewing might be an effective approach in maintaining the hippocampus-related spatial learning and memory. Chewing may represent a useful approach in pre- serving and promoting the hippocampus-dependent cognitive function in older people.
A full understanding of the mechanisms medi- ating mastication-induced hippocampal neurogenesis is complicated by the involvement of multiple regu- latory inputs, including stress hormone and its re- ceptor, neurotransmitter systems, memory-related genes and signaling pathways. Research to date has tended to focus on the contribution of the masticatory stimulation beneficial for hippocampal function. There is a paucity of information on how the multi- plicity of substrates, neurotransmitter systems and genes interact with one another to modulate the in- teraction between mastication and hippocampal function. Future studies should focus on cross-talk between neurotransmitter and receptor systems and adopt a neural networks approach to better under- stand hippocampal function. There is no doubt that the numerous animal models that have been devel- oped have facilitated an increased understanding the relationship between mastication and the hippocam- pal functional morphology. It may be worth consid- ering which of these animal models most appropri- ately models in humans. This is a complex considera- tion and it is very difficult to single out any one model as being the one that most closely models the human condition when the human condition itself is not yet fully understood. There is a need for further studies examining the association between mastication and the hippocampus- dependent cognition over the life-course, and the influence of aging on cognition, in animal models and humans.
This work was supported by a Grant-in-aid for Scientific Research from the Japan Society for the Promotion of Science to H.C. (No. 26462916).
The authors have declared that no competing interest exists.
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